Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K2933192:Design

Designed by: Jian Dong   Group: iGEM19_TJUSLS_China   (2019-09-16)


T7 promoter+RBS b+linker h+His+Linker a+Sumo+Linker b+JOHN-1+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 565
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 390
    Illegal NheI site found at 167
    Illegal NheI site found at 1244
    Illegal PstI site found at 565
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 390
    Illegal BglII site found at 279
    Illegal BamHI site found at 478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 565
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1089


Design Notes

None


Source

NCBI

References